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4ŒŽƒCƒ‰ƒXƒg ƒ‰ƒCƒ"-Jan 28, 13 · To produce apoRBP, add an equal volume of heptane to the purified holoRBP and mix gently by rotating overnight at 4 °C Centrifuge at 16,000 g for 10 min at 4 °C and carefully transfer the bottom aqueous phase to a new tube (avoiding the precipitate at the interface) Repeat the process three times with a 3 hr incubation for eachI c c W( Y U P) @ I ɔ A l A B Ȃǐ { ̒g n ̗щ ɐ 闎 t ؁B @ Ԋ ͔ӏt ŁA } 1 ̉ԉ肩 琔 t Ɠ 1 `3 ̉Ԃ ܂ B

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